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14 It also acts as an anti-proliferative agent and indices apoptosis by a) changing the mitochondrial membrane barrier, b) increasing reactive oxygen species, c) decreasing internal glutathione level , 76,77 d) increasing cytochrome-C activity, e) disturbing B-cell lymphoma-2 and B-cell lymphoma-2-associated X protein (Bcl/Bax) proportion, f) increasing caspase 3 and caspase 9 action, and g) poly ADP ribose polymerase

The following chemicals were obtained from Sigma-Aldrich: N-acetylcysteine (NAC), Tiron, Trolox, N-ethyl maleimide (NEM) and 4,6-Diamidino-2-phenylindole (DAPI)

Glutathione metabolism in lenses of dogs and rabbits: activities of five enzymes

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