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NAD+ Reconstitution by Vial Size Vial size 750 mg BAC water added 7.5 mL Final concentration 100 mg/mL Volume per 50 mg 0.50 mL (50 units) Volume per 100 mg 1.00 mL (100 units) Vial size 750 mg BAC water added 4.5 mL Final concentration 166.7 mg/mL Volume per 50 mg 0.30 mL (30 units) Volume per 100 mg 0.60 mL (60 units) Vial size 1,000 mg BAC water added 10.0 mL Final concentration 100 mg/mL Volume per 50 mg 0.50 mL (50 units) Volume per 100 mg 1.00 mL (100 units) Vial size 1,000 mg BAC water added 5.0 mL Final concentration 200 mg/mL Volume per 50 mg 0.25 mL (25 units) Volume per 100 mg 0.50 mL (50 units) Vial size 1,500 mg BAC water added 10.0 mL Final concentration 150 mg/mL Volume per 50 mg 0.33 mL (33 units) Volume per 100 mg 0.67 mL (67 units) Higher-concentration mixes reduce injection volume but require finer measurement on the syringe

Active metabolite characterisation whether smaller peptide fragments retain biological activity

Supplies Needed Quantities below assume an 816 week course of once-daily injections with gradual titration

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