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The D-CuP release efficiency was measured by UV-vis spectrophotometer at 600 nm

In order to investigate whether elevated levels of reduced GSH were due to less redox active heme iron being liberated from impaired hemoglobin uptake and digestion in resistant parasites, 24 hpi trophozoite-stage parasites were treated for 3 h with 10 M of the cysteine protease inhibitor, E64d, to block hemoglobin digestion and decrease free heme levels

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10.3389/fcell.2015.00080 75 PiacentiniS.LA FraziaS.RiccioA.PedersenJ